We have developed prototype software for cell segmentation and tracking of dual labelled Arabidopsis roots (nuclear and plasma-membrane markers) in 4-D. 

Nuclei are tracked located and tracked in 3-D versus time.  Cell vertices are identified and used to reconstruct cell networks, which are then tracked with time.  Software run from ImageJ.

Figure: Arabidopsis root dual-labeled with nuclear and cell membrane markers

For more information, please contact Stephen McKenna [1] (algorithms and coding), Glyn Bengough [2] (biophysical applications), Tracy Valentine [3] (microscopy and applications), Jim Haseloff [4] (plant material and cell biology).